FAQ List

How much DNA template do I need to give to BATJ?

BATJ recommends the following quantities for each sequencing reaction. Higher DNA concentrations typically result in stronger signal intensity.

PCR Product

Length	Recommended Quantity
200 – 500 bp	6 – 20 ng
500 – 1000 bp	10 – 40 ng
1000 – 2000 bp	20 – 80 ng
> 2000 bp	80 – 200 ng

Plasmid Template:

Type	Recommended Quantity
Single-stranded	150 – 300 ng
Double-stranded	300 – 800 ng (depending on insert and vector size)

We recommend doubling the above amounts in case a repeat sequencing reaction is needed.

What is required for premixed samples?

• 1. Do I have to provide primers (oligos)?

  1. BATJ provides common primers for free:

  T7	AAT ACG ACT CAC TAT AGG GAG
  T7 Terminator	GCT AGT TAT TGC TCA GCG G
  T3	ATT AAC CCT CAC TAA AGGGA
  SP6	GAT TTA GGT GAC ACT ATA G
  M13F (Universal)	TGT AAA ACG ACG GCC AGT
  M13F (-47)	CGCC AGG GTT TTC CCA GTC ACG AC
  M13R	CAG GAA ACA GCT ATG ACC
  pGEX Forward	GGG CTG GCA AGC CAC GTT TGG TG
  pGEX Reverse	CCG GGA GCT GCA TGT GTC AGA GG
  BGH Reverse	AAC TAG AAG GCA CAG TCG AGG
  pFBF	TAT TCC GGA TTA TTC ATA CC
  pFBR	CAA ATG TGG TAT GGC TGA TT
  CMV-Forward	CGC AAA TGG GCG GTA GGC

  2. Uncommon primers:

  Customers should provide uncommon primers at a concentration of 10 pmol. BATJ can dilute them if needed.
  BATJ also offers synthesis of primers for an additional charge.

• 1. For High-copy Number Plasmids:
  
• 2. For Low-copy or Very Low-copy Number Plasmids:
  

• 1. How do I place an order and schedule a pickup?

  Please download the request form for the desired service, fill it in, save it, and email it to:

  batjseq@gmail.com and/or service@batj.net

  Also, please give us a call in case we do not receive your email due to internet issues.

  Note: All orders (except PCR orders) placed by 4:00 PM local time will be picked up on the same day.

• 2. By what time do we need to submit PCR orders?

  To receive PCR sequencing results by the next day, please submit PCR orders before 12:00 PM (noon). These orders will be picked up at noon to allow time for PCR, clean-up, and sequencing.

• 3. Does BATJ accept mail-in orders?

  Yes. Orders from locations far from our base can be mailed via UPS or FedEx for timely delivery to:

  BATJ Inc
  6331 Nancy Ridge Dr.
  San Diego, CA 92121

• 1. How soon can I receive the results?

  Generally, results will be available the next day, no longer than 24 hours.

  For orders including services such as primer synthesis, sample purification, or primer walking, the turnaround time may vary depending on the service.

• 2. How will I receive the results?

  There are two delivery methods:

  • Small orders are sent via email.
  • Large orders may be transferred through FTP.
• 3. What format will the files be in?

  Sequencing results are provided as plain text files and electropherogram (.ab1) files.

• 4. Where can I get the necessary programs to read the sequencing data?

  If you don't have commercial software like ABI Sequencing Analysis, the following free tools are available:

  • Sequence Scanner – for Windows
  • Chromas – Windows (older version available for free)
  • Sequencher – demo version available for Mac and Windows
• 5. What is your repeat policy?

  As of 2017, BATJ does not offer free repeats by default. However, if a sequencing failure is suspected to be BATJ’s responsibility, we will reuse the same DNA and primer for a one-time repeat. If the issue persists, the additional attempt will be treated as a new order.

• 1. How long do you keep my samples?

  BATJ does not retain any customer sequencing data.

  We store DNA samples for one week in case a reaction needs to be repeated.

  If you would like us to retain your DNA or primers for future orders, please include a note in the “Comments” section of your order such as:

  “Please keep my DNA, primer, or both for future orders.”

  In such cases, we will store your DNA and/or primers for up to one month.