RC Cloning Kit is a new cloning method with the recombinase cocktail technology (in patterning process by BATJ).
RC Cloning Kit provides an easy and robust method to clone one or multiple PCR fragments into the desired vector seamlessly. There is no need to either design compatible cohesive restriction ends as in traditional cloning methods or employ a cloning shuttle.
As shown in figure on protocal, the desired vector is linearized by using the restrict enzymes and the interested DNA sequence is amplified with PCR by using the primer carrying extra twenty nucleotides homologous to the two open ends of the vector backbone. Incubation of the linearized vector and PCR fragments with the recombinase cocktail results in circulated plasmid bearing interested PCR product for transformation.
The results demonstrate that the RC Cloning Kit is capable of cloning the PCR product up to 8kb.