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1. DNA Sequencing Services:

Long range (LR) DNA sequencing done with the newest version of the ABI 3730 capillary machine, readable bases up to 1,000 and longer. DNA sequencing price depends on the service.
Turnaround time is 24 hours for most sequencing. Free pick up is offered for local companies, if the order is over five reactions.
Quality Guarantee: If desired, one free sequencing repaet is offered per reaction.
Sequencing trace data and/or text files will be sent via email.

File Download:
(1). For your convenience, a Sequencing Request Form is avaliable in Excel Format. It can be downloaded for easy editing at

"Sequencing Request Form-Excel version"

(2). To read DNA sequencing Chromatogram files
** Sequencher has a free demo version for MAC and Windows.
** Chromas for Windows platforms. An older version of the program is free for download.

2. DNA Preparation Service:

DNA mini-prep with column (Qiagen) or gel clean method with Mini-Prep 96 instrument (MacConnell). The instrument uses a revolutionary new method of nucleic acid purification based on gel electrophoresis and subsequent nucleic acid recovery by electroelution. It is the best method for high throughput (96 samples each time). The resultant DNA is highly pure (95% supercoil) and can be used for DNA sequencing, restriction digests, transformations and transfections.
*Prices: DNA mini-prep with column: $2/each; DNA mini-prep with machine: $3/each
**The gel clean method includes colonies pickup from customer plates, culture with our Low-salt TB, and DNA purification with machine. Over 24 samples only please.
Normal or Endo-free MIDI, MAXI or MEGA DNA preparation with high qualified DNA prep (Qiagen) kits. Endo-free products are less than the 0.1 EU/ug by LAL detection
BATJ provides DH5α as a standard host strains for DNA prep. If your DNA requires special hosts, please provide the bacterial colony or glycerol stock as starting material. We will perform the transformation step if necessary.
Provide 100ng of plasmid DNA or fresh colonies on LB plate with plasmid information, such as antibiotic resistance and copy number, then mail or send your request to BATJ Inc.
Turnaround time: 3-4 days
Prices: Midi: $50(Endo-free $80);MAXI:$150(Endo-free $180) MEGA: $250(Endo-free $300）GiGA:$699(Endo-free $799)
*For large scale, the price of Endo-free DNA prep is $100/mg

3. Mutagenesis:

Site-directed mutagenesis for any combination of mutation within a 4 base-frame. The price includes quick change reaction, transformation, mini-prep and sequencing verification.
Turnaround time is 3 to 5 workdays in most cases.
DNA template and identified sequencing data will be sent back to customer.
When ordering, email the original and modified sequence of the construct to "service@batj.net" indicating the specific base(s) changes. Please provide plasmid information such as antibiotic resistance and the sequencing primer for sequencing confirmation. Please call to arrange pick up of the plasmid DNA.
Prices: For each mutagenesis is $299.
* If do more than one change is desired, call us for discount pricing.

4. Primer Synthesis:

BATJ offers high quality longer oligos (up to 120 bases) for gene synthesis, cloning, mutagenesis quick change, primer walking sequencing, and PCR applications. Our synthesis machine has a real time trityl monitor which guarantees product quality and no lost bases during synthesis. Primer prices depend on the scale, number of bases, and purification level. Turnaround time is normally 24 hours, two additional days for PAGE purification and HPLC purification.

5. Cloning and subcloning Service:

Clone an insert of interest into a vector provided by the customer. The cloned gene will be sequenced upon completion for verification. Sequencing data and mini-prep will be sent back to the customer. Other prep services are available at additional cost.
Please send vector and insert information such as the size, restriction enzyme sites, antibiotic resistance, etc. via email to service@batj.net. For more information or for pick-up service, please call us.
Prices: Cloning is $599; Subcloning is $499
* If do more than one cloning service is desired each time, call us for discount price.

6. Protein Expression (Bacterial):

Cloning into Expression pET Vector:
Clone (subclone) interest gene into a pET expression vector. The cloned gene will be verified by sequencing. The final editing sequence data and mini-prep DNA will be sent back to the customer.
Please provide 1 ug of containing the gene of interest plasmid DNA and complete vector sequence in electronic format.
Identification of Expression Positive Clones:
Transfer pET plasmid containing the gene into a BL21 (DE3).Over-express target small scale protein. Test recombinant protein by SDS-PAGE and western Blot (customer must provide appropriate antibodies).
Please provide 1 ug of pET plasmid containing interest gene and complete vector sequence in electronic format.
Large-scale Culture:
Make one liter or more of bacterial culture induced with an IPTG inducer and harvest cell pellet for protein purification.
Please provide expression positive clone(E.coli glycerol stock or plate)

7. LAL Endotoxin (Pyrogen) Detection :

1. LAL Assay set up fee is $30: Set up fee includes preparation of positive control, negative control and inhibitor control. Our assay standard is 0.1 EU/ug, (Qiagen Endofree products is less than the 0.1 EU/ug), but LAL assay sensitive is from 0.03 EU to 2.0 EU depending on the customer requirements.
2. $20 for each LAL reaction. For each sample, we recommend that LAL assays are performed with the following concentration: (1) 0.05 EU/ug (2) 0.1 EU/ug (3) 0.5 EU/ug
Please provide 5-10 ug (Concentration > 200 ng/ul) of DNA sample for each reaction. Free pick up from local companies and labs. Results will be sent to you via e-mail. Turn around time is less than 24 hours.

8. Gene Synthesis Service:

BATJ Inc.'s excellent protocol for gene synthesis allows us to clone synthetic double stranded DNA into a plasmid within a very small timeframe. The high quality and low prices of BATJ's services enable us to serve a variety of biotech, research, pharmaceutical companies and institutions that seek the best possible results. Details in Gene synthesis information.