File And Software Downloads

• 1, Sequencing Request Form "Sequencing Request Form-Excel version".
• 2, Lifetech (ABI) has a program that is called 'Sequence Scanner' for Windows platforms.
• 3, Technelysium Pty Ltd has a product called Chromas, for Windows platforms. An older version of the program is free for download.
• 4, Gene Codes Corporation has a demo of their "Sequencher" program available for many platforms. However, the demo version lacks some features.

Easy ways to get answers:


• Call Phone: 858-622-9498
  
• E-mail:service@batj.net
  

About Samples

Q: How much DNA template do I need to give to BATJ?

BATJ recommends the following quantities for each sequencing reaction. However, note that high DNA quantities give higher signal intensities.

Template	Quantity
PCR Product:     200 - 500 bp     500 - 1000 bp     1000 - 2000 bp     >2000 bp	6 - 20 ng 10 - 40 ng 20 - 80 ng 80 - 200 ng
Single-stranded	100 - 200 ng
Double-stranded	200 - 600 ng (depends on the size of the insert and vector)





A doubling of the above amount is also recommended, in case the sequencing reaction needs to be repeated due to unforeseen circumstances.




Q: What is the required for premixed samples?

For premixed sequencing service, please mix templates and primer as follows:
1.In 5 µl premix, please add 350 ng of ds DNA, or 40 ng of cleaned PCR products.
2.In this 5 µl, please add 3.2 pmol ( about 20 ng) of primer.
3.Please leave a note to indicate your samples are premixed.


About Primers

Q: Do I have to provide primers (oligos)?

1. BATJ provides common primers for free (see below)

T7	AAT ACG ACT CAC TAT AGG GAG
T7 Terminator	GCT AGT TAT TGC TCA GCG G
T3	ATT AAC CCT CAC TAA AGGGA
SP6	GAT TTA GGT GAC ACT ATA G
M13F (Universal)	TGT AAA ACG ACG GCC AGT
M13F (-47)	CGCC AGG GTT TTC CCA GTC ACG AC
M13R	CAG GAA ACA GCT ATG ACC
pGEX Forward (GST 5, pGEX 5')	GGG CTG GCA AGC CAC GTT TGG TG
pGEX Reverse (GST 3, pGEX 3')	CCG GGA GCT GCA TGT GTC AGA GG
BGH Reverse	AAC TAG AAG GCA CAG TCG AGG
pFBF	TAT TCC GGA TTA TTC ATA CC
pFBR	CAA ATG TGG TAT GGC TGA TT
CMV-Forward	CGC AAA TGG GCG GTA GGC

2. Customers should provide uncommon primers at a concentration of 10 pmol (however, BATJ can dilute the concentration). If needed, BATJ could synthesize primers for our customers for additional charge.


About Order and Sample Pickup

Q: How do I place an order and schedule a pickup?

• Login to your account from "http://www.batj.net/users/login.php", click "Place Order" and fill in the order form with your sample information, then submit your order. To confirm your order, please give us a call, in case we do not receive your order because internet problem.
• Download the "Sequencing Request Form "Sequencing Request Form-Excel version", fill it in, then email the form to "order@batj.net"
• All orders placed by 4:00pm in local will be picked up on the same day.
  

  Q: Does BATJ accept mail-in orders?

  Orders from areas too far from our base of operations should be mailed through UPS or FedEx for timely delivery to:
  

  BATJ Inc
  6042 Cornerstone Ct. Suite E
  San Diego, CA 92121
  

  About Results

  Q: How soon can I receive the results?

  Generally, results will be available the next day, no longer than 24 hours.
  
  For orders with other service items, such as primer synthesis, sample purification, or primer walking etc., it may take a longer time depending on the specific services requested.

  
  

  Q: How will I receive the results?

  There are two ways:
  Small orders will be sent to you through email.
  Order with a large amount of data could be transferred through internet by “FTP”.
  
  

  Q: What format will the files be in?

  BATJ’s sequencing programs generate text files and electrophoregram files, either or both of which may be delivered to our customers.

  
  

  Q: Where can I get the necessary programs to read the sequencing data?

  If you do not have a commercial program (ABI Sequencing Analysis, for example) to read electrophoregram files, there are a number of free software programs that are available to open the files, please select one based on your computer platform and operating system.
• Lifetech (ABI) has a program that call "Sequence Scanner" for Windows platform.
• Technelysium Pty Ltd has a product called "Chromas", for Windows platforms. An older version of the program is free for download.
• Gene Codes Corporation has a demo of their "Sequencher" program available for many platforms. However, the demo version lacks some features.




Q: What is your repeat policy?

Except for premixed samples, regardless of the cause of failure, if the results are unsatisfactory and a repeat requested, we will provide a free one-time repeat. However, the original reaction will be charged. In other words, we charge once and only once for both the original and repeat reactions.


Other

Q: How long do you keep my samples?

BATJ is a biotech service company, we do not keep any customer's sequencing data. We only keep your DNA samples for one week, in case a reaction needs to be repeated.
If you need either DNA or primers for future orders, please specify this in the “Comments” section of your order. Just write “Please keep my DNA, primer, or both for future orders.” We can store your DNA and/or primers for one month.